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通用引物

引物名称 序列
pmirGLO-T5-seqF GGACGAGGTGCCTAAAGGAC
pGL3-T5-seqR(-100) gCCTTATgCAgTTgCTCTCC
pGL3-T5-seqF(-100) ACTAACATACGCTCTCCATC
Pet28a-T5-seqR ACCCCTCAAGACCCGTTTAG
PCDH130-seq-R CACCCGTTCAATTGCCGACC
pLVmini-seq-R AGCAGATCTTATCTTCGTTG
YM017 CATTTTCAgggATAgCAAgC
YM016 TCACACAggAAACAgCTATg
YFP-NC AgATCTggCggAggAggTTC
YFP-cc ggATCCCATTgTACAgCTCgTCCATgCC
WPRE-R ggCATTAAAgCAgCgTATCC
ubic-r TTCgTCTgACgTggCAgCgCT
ubic-f TgTACCTATCTTCTTAAgTAgCTgAAgCTCC
Ubi TTgTCgATgCTCACCCTgTTg
U6 ATggACTATCATATgCTTACCgTA
T7ter gCTAgTTATTgCTCAgCgg
T7 eev promotor AAggCTAgAgTACTTAATACgA
T7 TAATACgACTCACTATAgg
T3 ATTAACCCTCACTAAAgggA
SV40-promoter gCCCCTAACTCCgCCCATCC
SV40-PArev CCTCTACAAATgTggTATgg
SV40-CX-L(chimeng) TTTTATgTTTCAggTTCAgg
Sq-FP-chimeng gAAggTTgTggATCTggATA
SP-Rzengdongchang TgCAATAACTTCgTATAggC
Sp-L1zengdongchang gCggTgTCATCTATgTTACTAg
SP6 ATTTAggTgACACTATAg
shpDown-F AgCTACATTTTACATgATAggCTT
SFB-RP ATTTAgCAgCAgCggTTTCT
SFB-FP gTCCACTCCCAggTCCAACT
SeqF-chimeng ATTTTTgTgATgCTCgTCAg
S.tag CgAACgCCAgCACATggACA
RVP4 gACgATAgTCATgCCCCgCg
RVP3R CgTCTTCgAgTgggTAgAATg
RVP3 CTAgCAAAATAggCTgTCCC
RV-M gAgCggATAACAATTTCACACAgg
RREUP(chen) CTgCTgCTgCACTATACCAgA
RREdown(chen) TCTggTATAgTgCAgCAgCAg
RFP-5-primer TACTCAgATCTCgATATCgAA
RFP-3-primer gATCAgTTATCTAgATCCggT
pYES2-F TAATATACCTCTATACTTTAACgTC
pYES2.R TCggTTAgAgCggATgTg
PX330-SEQ-F TATTTCCCATgATTCCTTCATATTTgC
pUp-pDown-flank-r AATACgACTCACTATAggggA
pUp-pDown-flank-f CggCCAgTCTTAAgCTCggg
PUC57-seq-R CTggCACgACAggTTTCCC
pUC57-seq-F ACTgTTgggAAgggCgATC
PUC57-PGK-SEQ-R ACCggTggATgTggAATgTgTg
PUC57-PGK-SEQ-F CATTCTgCACgCTTCAAAAgCg
pUC510R TTCCggCTCgTATgTTgTgT
pUC344 gTgCTgCAAggCgATTAAgT
PUAST gCTCACCTgTgATTgCTCCT
Psin-Ef1a(panzu) AgTTgCgTgAgCggAAAgATgg
Psicheck2.R gCCgCCTCCgAATgAgAgTg
Psicheck2.F TgAAgggCCTCCACTTCAgC
pSG5-3 gAAATTTgTgATgCTATTgC
PQE30R gTTCTgAggTCATTACTgg
PQE30F TgAgCggATAACAATTTCAC
PMX-PR TTATCgTCgACCACTgTgCTg
PMX-PF gACggCATCgCAgCTTggAT
PMSCV-5 CCCTTgAACCTCCTCgTTCgACC
PMSCV-3 gAgACgTgCTACTTCCATTTgTC
pMAL-C2X-R CTgCAAggCgATTAAgTTgg
pMAL-C2X-F TgCgTACTgCggTgATCAAC
pLXSN-R gTTgCTgACTAATTgAgATg
pLXSN-F CTTgAACCTCCTCgTTCgAC
plv-final-internal-r AgCCTgCTTTTTTgTACAAACTTg
plv-final-internal-f CAAgTTTgTACAAAAAAgCAggCT
pLV(KD)-Puro-seq-PF3 gTggATCTCTgCTgTCCCTgTAAT
pLNCX-R ACCTACAggTggggTCTTTCATTCCC
pLNCX-F AgCTCgTTTAgTgAACCgTCAgATC
pLNCX2-R ACCTACAggTggggTCTTTCATTC
pLKO-EGFP-seqR gTTTACCgTAAATACTCCAC
plko.1.seqR gCggCCAAAgTggATCTCTg
pLKO.1 puro-seq-PF(62.1) gAgggCCTATTTCCCATgATTC
plenti-seq-R CTTTACATggTCggAAATgC
plenti-seq-F CCgCTTCCTTCTACATgTTACC
pl-cn-R CACgACATTCAACAgACCT
pl-cc-R CACATTgCCAAAAgACgg
pJET1.2 reverse AAgAACATCgATTTTCCATggCAg
pJET1.2 forward CgACTCACTATAgggAgAgCggC
PITES2-EGFP-5 gTAggCgTgTACggTgggAg
PIRES3-EGFP-R AACgCACACCggCCTTATTC
pGL3-T5-seqR CTTATgCAgTTgCTCTCCAg
pGL3-T5-seqF gCgggCCTCTTCgCTATTAC
PGK-FP CCTTCgCTTTCTgggCTCA
PGK-charu-R(panguangjinzu) ACCggTggATgTggAATgTgTg
PGK3 ACTTgTgTAgCgCCAAgTg
PGEX-F TATTCTgAAATgAgCTgTTgAC
PGEX-5 gggCTggCAAgCCACgTTTggTg
PGEX-3 CCgggAgCTgCATgTgTCAgAgg
pGAP-F(nie) gTCCCTATTTCAATCAATTgAA
pFastBacR CAAATgTggTATggCTgATT
pFastBacF ggATTATTCATACCgTCCCA
pET-R gCTAgTTATTgCTCAgCgg
pET-F2 TggCCAggACCCAACgCTgC
pET-F ATgCgTCCggCgTAgAggAT
pEGFP-N-5 TgggAggTCTATATAAgCAgAg
PEGFP-N-3(EGFP-Nrev) CgTCgCCgTCCAgCTCgACCAg
pEGFP-C-5(EGFP-cfor) CATggTCCTgCTggAgTTCgTg
pEGFP-C-3 TATggCTgATTATgATCAgT
pEAQ-R ACTCTgAgCACAgAAAACCg
pEAQ-F TgTTACgATTCTgCTgACTTTC
PDONR-R gTAACATCAgAgATTTTgAgACAC
PDONR-F TCgCgTTAACgCTAgCATggATCTC
PDC315-R CgATgCTAgACgATCCAg
PDC315-F ACgTgggTATAAgAggCg
Pcold-R TggCAgggATCTTAgATTCTg
Pcold-F ACgCCATATCgCCgAAAgg
PCMV-R TCCAAACTCATCAATgTATC
PCMV-F TCTAAAAgCTgCggAATTgT
PCMV6.F TTgTTggTCATgCggCCCTg
pcDNA3.1-T5-R CCAgggTCAAggAAggCACg
PCDNA3.1R(BGH) TAgAAggCACAgTCgAgg
PCDNA3.1F CTAgAgAACCCACTgCTTAC
pCDH-EF1a-R(xiechen) ACACgACATCACTTTCCCAg
pCAGGS-seqR AAAATTCCAACACACTATTgCAATg
pCAGGS-seqF AgAgCCTCTgCTAACCATgTTCATg
pB-XhoI-seq-FP TgAACACgTCgCTAAgCgAA
pB-XbaI-seq-RP gCCTCATCATCACTAgATgg
PBV220R CTgCgTTCTgATTTAATCTg
PBV220F AAgAAgggCAgCATTCAAAg
pBD-GAL4-R AAgAgTTACTCAAgAACAAgAA
pBD-GAL4-F gCCTCTAACATTgAgACAgC
pBAD-R1 AACAgCCAAgCTggAgACC
pBAD-R gATTTAATCTgTATCAgg
pBAD-F1 TTTATCCATAAgATTAgCgg
pBAD-F ATgCCATAgCATTTTTATCC
pBaBe-5 CTTTATCCAgCCCTCAC
pBaBe-3 ACCCTAACTgACACACATTCC
OpIE2Reverse gACAATACAAACTAAgATTTAgTCAg
OpIE2Forward CgCAACgATCTggTAAACAC
NS8 TCCgCAggTTCACCTACggA
NS1 gTAgTCATATgCTTgTCTC
NOS ACCggCAACAggATTCAATC
NA-F(dongyangguang) gCgCCACCAgACATAATAgC
MT-forward CATCTCAgTgCAACTAAA
Mouse-u6 CAgCACAAAAggAAACTCACC
MO2.F CAgCCTCCggACTCTAgC
mfe-seq TTggAggTCgCTgAgTAgTg
mcherry-R AgggAggAgTCCTgggTCAC
malEprimer ggTCgTCAgACTgTCgATgAAgCC
M13R(-48) AgCggATAACAATTTCACACAggA
M13R CAggAAACAgCTATgACC
M13F(-47) CgCCAgggTTTTCCCAgTCACgAC
M13F TgTAAAACgACggCCAgT
Lig3P3(chen) CAggCTAAgTTgACAACCACT
LentiD10A-F-chimeng CAgTACAATCTgCTCTgATg
ITS5 ggAAgTAAAAgTCgTAACAAgg
ITS4 TCCTCCgCTTATTgATATgC
ITS1 TCCgTAggTgAACCTgCgg
IRESrev CCTCACATTgCCAAAAgACg
hybirdintronF(chen) TgTAATTAgCTgAgCAAgAg
HIV-1up(chen) CgAgTCCTgCgTCgAgAgAg
hGH-pA-R CCAgCTTggTTCCCAATAgA
H1primer CgCTATgTgTTCTgggAAAT
GLP2 CTTTATgTTTTTggCgTCTTCCA
GLP1 TgTATCTTATggTACTgTAACTg
GAL4ADfor TACCACTACAATggATg
FUW-seq-F CgggTTTATTACAgggACAgC
F86-CX-L-chimeng ACCTggCCATCTCTgACCTg
espcas-down-F(chen) CACggATCgACCTgTCTCAgC
EFla TCAAgCCTCAgACAgTggTTC
EF1A-F2(panzu) CACTgAgTgggTggAgACTgAAgTTAg
EF1A-F(zibei) CTgggAAAgTgATgTCgTgT
EF13 CCAACTTCTCggggACTgTg
EBV-R gTggTTTgTCCAAACTCATC
DuetUP2 TTgTACACggCCgCATAATC
DuetDOWN1-Primer gATTATgCggCCgTgTACAA
DsRed1-N gTACTggAACTggggggACAg
DsRed1-C AgCTggACATCACCTCCCACAACg
CVN ggTgggAggTCTATATA
CMV-R gTTCACggTgCCCTCC
CMV-F TgggCggTAggCgTgTACggTg
CMV-enhancer-R-chimeng CTATTggCgTTACTATTgAC
CMV-30 ATAACCCCgCCCCgTTg
CMV-24 TTAggACAAggCTggTgg
CAGR2 gTAACgCCAATAgggACTTTC
BlaRV ACTCACgTTAAgggATTTTggTCA
BlaM1 AAgCCCTCCCgTATCgTAgTTATC
attB2-LRR ACCACTTTgTACAAgAAAgCTgggT
attB1-LRF ACAAgTTTgTACAAAAAAgCAggCT
a-Factor TACTATTgCCAgCATTgCTgC
ACYCDuetUP1 ggATCTCgACgCTCTCCCT
AC5 ACACAAAgCCgCTCCATCAg
AB-R(aisimai) CACTgCATTCTAgTTgTgg
AB-R CAAACTCATCAATgTATCTTATCA
AB-F TCTACgTCTTTgTTTCgT
AAVS1-Seq-R(group pan) CAggTAAAACTgACgCACggAgg
AAVS1-Seq-F(group pan) TTTCCggAgCACTTCCTTCTCg
601SaF-F-chimeng TTCCTTgACCCTggAAggTg
5BD TCATCggAAgAgAgTAgT
5AOX1 gACTggTTCCAATTgACAAgC
5AD AgggATgTTTAATACCACTAC
3BD TAAgAgTCACTTTAAAATTTgTATAC
3AOX1 ggCAAATggCATTCTgACAT
3AD AgATggTgCACgATgCACAg
35S gACgCACAATCCCACTATCC
27F AgAgTTTgATCCTggCTCAg
-20 TgTgTAgCgCCAAgTgCCCA
1525R AgAAAggAggTgATCCAgCC
1492R ggTTACCTTgTTACgACTT
(huijun)BGH-R gAAAggACAgTgggAgTg
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